define overlap between hot/cold spots of two variables

Given two numeric variables, this function will define hot and cold spots for each variable, and it will annotate cells/spots according to the presence/absence of overlap between the two hot/cold spot annotations. If a hot/cold spot annotation for one or both variables already exist,hotspot analysis will not be rerun for that variable(s)

hotspot_overlap(seurat = NULL, feat.1 = NULL, feat.2 = NULL, ...)

Arguments

seurat: a Seurat object containing Kandinsky data (`KanData()`)
feat.1: character string specifying the name of the first feature to consider
feat.2: character string specifying the name of the second feature to consider
...: arguments passed to hotspot_analysis function

Value

updated Seurat object with a new annotation column reporting the type of overlap between hot/cold spot annotations: - 'Hot-Hot': a cell/spot is annotated as 'hot' for both the considered variables - 'Cold-Cold': a cell/spot is annotated as 'cold' for both the considered variables - 'Cold-Hot': a cell/spot is annotated as 'cold' for one variable and 'hot' for the other - 'Hot': a cell/spot is annotated as 'hot' only for one of the two variable (the other is annotated as non-significant 'NS') - 'Cold': a cell/spot is annotated as 'cold' only for one of the two variable (the other is annotated as non-significant 'NS') - 'NS': a cell/spot is not annotated as either 'hot' or 'cold' for any of the two variables